Table 2.
Quantitative overview of trabecular bone in distal femur and proximal tibia using micro-CT analysis in mice treated with intranasal saline, 12.5% organic dust extract (ODE), or 100 ng lipopolysaccharide (LPS) for 3 weeks while maintained on low versus high vitamin D (VD) diet.
| Parameter | Saline Low VD |
Saline High VD |
ODE Low VD |
ODE High VD |
LPS Low VD |
LPS High VD |
p- value |
Mean % delta of Low vs. High VD |
||
|---|---|---|---|---|---|---|---|---|---|---|
| Saline | ODE | LPS | ||||||||
| Femur | ||||||||||
| Bone mineral density, mg/mm3 | 317 ± 3.05 | 330 ±3.53# | 304 ± 2.20* | 320 ± 7.30# | 309 ± 4.41 | 332 ± 6.62# | 0.001 | 4.10 | 5.26 | 7.44 |
| Bone volume, % | 8.54 ±0.20 | 9.22 ±0.25 | 7.50±0.15* | 8.63±0.51# | 7.40±0.34* | 9.65±0.55# | 0.001 | 7.96 | 15.06 | 30.40 |
| Specific bone surface, mm−1 | 102.7±1.5 | 101.3±1.2 | 105.4±2.5 | 101.7±2.9 | 114.7±2.2*† | 99.9±5.3# | 0.006 | −1.43 | −3.50 | −12.88 |
| Trabecular pattern factor, mm−1 | 29.15±0.55 | 27.95±0.47 | 30.74±0.64 | 29.16±1.26 | 31.69±0.84* | 26.57±1.5# | 0.006 | −4.12 | −5.12 | −16.16 |
| Average object area/slice, µm−2 | 2700 ± 80 | 2800 ± 80 | 2500 ± 110 | 2700 ± 140 | 2100 ± 90* | 2900±330# | 0.010 | 3.70 | 8.00 | 38.10 |
| Trabecular thickness, µm | 35.4 ± 0.75 | 35.1 ± 0.32 | 34.6 ± 1.0 | 35.3 ± 1.0 | 31.9 ± 0.44 | 37.2 ± 2.23 | 0.054 | −0.85 | 2.02 | 16.61 |
| Trabecular number, µm−1 | 242 ± 37 | 263 ± 61# | 217± 28* | 243 ± 87# | 232 ± 83 | 260 ± 58# | 0.001 | 8.68 | 12.00 | 12.07 |
| Trabecular separation, µm | 202 ± 2.33 | 195 ± 3.40 | 211 ±2.24* | 201 ± 3.0# | 201 ± 3.44 | 194 ± 2.93 | 0.011 | −3.47 | −4.74 | −3.48 |
| Tibia | ||||||||||
| Bone mineral density, mg/mm3 | 294 ± 3.70 | 303 ±2.13# | 287 ± 1.71 | 299± 4.80# | 282 ± 3.10* | 306± 6.50# | 0.001 | 3.06 | 4.18 | 8.51 |
| Percent bone volume, % | 6.16 ± 0.27 | 6.82±0.16# | 5.64 ± 0.26 | 6.67±0.28# | 5.65 ± 0.30 | 7.30±0.42# | 0.002 | 10.71 | 18.26 | 29.20 |
| Specific bone surface, mm−1 | 111.4±2.4 | 107.5±1.2 | 115.9±2.4 | 111.1±2.0 | 111.7±2.4 | 101.5±4.0#† | 0.011 | −3.55 | −4.12 | −9.17 |
| Trabecular pattern factor, mm−1 | 36.07±1.15 | 34.01±0.42 | 36.61±0.62 | 33.94±1.05 | 36.67±1.05 | 33.06±1.11 | 0.054 | −5.71 | −7.30 | −9.84 |
| Average object area/slice, µm2 | 1700 ± 90 | 1800 ± 40 | 1500 ± 40 | 1700 ± 60 | 1700 ± 70 | 2000 ± 30 | 0.034 | 5.88 | 13.33 | 17.65 |
| Trabecular thickness, µm | 44.2 ± 0.8 | 44.3 ± 0.5 | 45.2 ± 1.2 | 43.7 ± 0.9 | 43.4 ± 0.7 | 47.5± 2.0 | 0.152 | 0.23 | −3.32 | 9.45 |
| Trabecular number, µm−1 | 140 ± 50 | 154 ± 42# | 125 ± 73 | 153 ± 71# | 130 ± 63 | 154 ± 65# | 0.004 | 10.00 | 22.40 | 18.46 |
| Trabecular separation, µm | 233 ± 3.70 | 230 ± 4.25 | 232 ± 6.73 | 219 ± 3.47 | 253 ± 5.48*† | 225± 6.78# | 0.007 | −1.29 | −5.60 | −11.07 |
Mean ± SEM of treatment groups with N=4–8 mice/group combined from independent studies. P-value denotes statistical significance (ANOVA) among treatment groups.
Statistical difference between groups denoted by asterisks (* p<0.05) as compared to respective saline,
number sign (#) compares respective low vs. high vitamin D,
dagger sign († p<0.05) compares respective ODE and LPS.