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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: DNA Repair (Amst). 2015 Jan 31;29:16–22. doi: 10.1016/j.dnarep.2015.01.008

Fig. 4.

Fig. 4

Processive DNA synthesis by hPolε and p261N on singly-primed M13mp2 ssDNA templates at 20 °C. A pre-incubated solution of hPolε or p261N (250 nM) and 5′-radiolabeled (A) 21- or (B) 45-mer primer annealed to M13 ssDNA (25 nM) was mixed with all four dNTPs (100 μM) for various times before quenching with the addition of 0.37 M EDTA. Products extended from the 21-mer primer were separated by 17% denaturing PAGE, while products extended from the 45-mer primer were separated by 8% denaturing PAGE.