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. 2015 Feb;13(2):85–92.

Figure 1.

Immunohistochemical expression of germ cells applying polyclonal antibody against bax (A-C).

Figure 1

(A): Control; The germ cells present immunolabeling for BAX primarily in spermatocytes (arrows) showing no evidences in spermatogonia. (B): Saline on day 7; Intensive BAX cytoplasmic expression in high population of spermatocytes, whereas the spermatogonia are more resistant to anti-BAX protein and therefore to apoptosis. (C): Ghrelin on day7; only few spermatocytes are showing cytoplasmic deep-brown staining against apoptotic marker (A-C) (400×).

Gc: Giant cell

Lc: Leydig cell

Sc: Sertoli cell

Sg: Spermatogonia

Sp: Spermatocyte