Figure 4.

Western blot of L₂TES with INP-1E4G4C2 MoAb Electrophoresis was performed on 4–20% polyacrylamide slab gradient gels former and separated at 150 V for 2 h. Proteins were transferred to a PVDF membrane immobilon. Nonspecific sites were blocked overnight with 5% skimmed milk in PBS-tween 20 at 4°C, incubated 2 h with INP-1E4G4C2 MoAb at room temperature and washed three times. Added and incubated for 2 h at room temperature with anti-mouse IgG-HRP conjugate (diluted 1:1,000) and washed three times; the substrate/chromogen solution was added and the reaction was stopped with distilled water. (M) kaleidoscope prestained standards. (1) 100 μg L₂TES/well.