Skip to main content
. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2015 Mar 26;35(5):1198–1206. doi: 10.1161/ATVBAHA.114.305185

Figure 3. Smads and Nox4 mediate Hsp27 upregulation by TGFβ.

Figure 3

Figure 3

Figure 3

HASMCs were transfected with siNeg, siNox4 (A) or siSmad4 (C) or treated with NAC (B) and maintained in serum-free medium for 2 days prior to TGFβ treatment (2 ng/mL) for 24 h. Total proteins were extracted and subjected to western blot analysis for Hsp27 and Smad4. Tubulin was used as a loading control. Bars represent the average of four independent experiments ± SEM.