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. Author manuscript; available in PMC: 2016 May 1.

Published in final edited form as: Arterioscler Thromb Vasc Biol. 2015 Mar 26;35(5):1198–1206. doi: 10.1161/ATVBAHA.114.305185

Quiescent HAMSCs were treated with non-silencing RNA (siNeg) or siRNA against Hic-5 (siHic-5) for 2 days. After treatment with TGFβ (2 ng/mL) for 24 h, cells were fixed and stained for Vinculin (red), and nuclei were detected with DAPI (blue). Scale Bar = 20 μm. (A). The image represents the average of the FA number per cell, n=25. Four independent experiments were performed with consistent results (B).

Quiescent HAMSCs were treated with non-silencing RNA (siNeg) or siRNA against Hic-5 (siHic-5) for 2 days. After treatment with TGFβ (2 ng/mL) for 24 h, cells were fixed and stained for Vinculin (red), and nuclei were detected with DAPI (blue). Scale Bar = 20 μm. (A). The image represents the average of the FA number per cell, n=25. Four independent experiments were performed with consistent results (B).