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. Author manuscript; available in PMC: 2016 May 1.

Published in final edited form as: Arterioscler Thromb Vasc Biol. 2015 Mar 26;35(5):1198–1206. doi: 10.1161/ATVBAHA.114.305185

Immediately after siRNA electroporation, HASMCs were seeded onto fibronectin-coated glass coverslips. One day post-seeding, the cells were serum-starved for 48 h, stimulated with 2 ng/mL of TGF-β for 24 h, and spun inside the spinning disk device to quantify adhesion strength, which is defined as the shear stress at which 50% of cell detachment occurs. Representative adhesion profiles for each of the each condition (A) and bar graph is the average of n=7–10 (B). Hic-5 deficient cells have impaired cells migration (C). After transfections, HASMCs were starved for 48 h, trypsinized, and reseeded in a collagen-coated Transwell insert. After stimulation for 3 h with PDGF (10 ng/mL), the cells migrating through the membrane were stained with DAPI and counted. The graph represents mean±SEM of migrated cells per field from 3 independent experiments. *P<0.01.

Immediately after siRNA electroporation, HASMCs were seeded onto fibronectin-coated glass coverslips. One day post-seeding, the cells were serum-starved for 48 h, stimulated with 2 ng/mL of TGF-β for 24 h, and spun inside the spinning disk device to quantify adhesion strength, which is defined as the shear stress at which 50% of cell detachment occurs. Representative adhesion profiles for each of the each condition (A) and bar graph is the average of n=7–10 (B). Hic-5 deficient cells have impaired cells migration (C). After transfections, HASMCs were starved for 48 h, trypsinized, and reseeded in a collagen-coated Transwell insert. After stimulation for 3 h with PDGF (10 ng/mL), the cells migrating through the membrane were stained with DAPI and counted. The graph represents mean±SEM of migrated cells per field from 3 independent experiments. *P<0.01.