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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Hum Pathol. 2015 Mar 10;46(6):876–883. doi: 10.1016/j.humpath.2015.02.015

Table 1.

Protocols for immunohistochemistry*

Antibody Vendor Titer Time Epitope Retrieval Method of Detection
ER BioCare 1:1000 30 min BioCare Nuclear Decloaker Envision + LP, mouse (Dako)
PR Dako 1:5000 30 min Citrate, pH6 Envision + LP, mouse (Dako)
Ki-67 Dako 1:1000 30 min BioCare Reveal Envision + LP, mouse (Dako)
HercepTest Dako P.D. Her2 30 min Per Kit Instructions Kit Components (K5204, Dako)
Transgelin (Anti SM22 alpha antibody) (ab14106) Abcam 1:3000 30 min Biocare Envision + LP, mouse (Dako)
*

All stains were performed using the Dako Autostainer per manufacturer procedures. Buffer used was Tris-buffered saline with Tween; visualization with Dab+ (Dako)