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. Author manuscript; available in PMC: 2016 Jun 1.

Published in final edited form as: Am J Ophthalmol. 2015 Feb 19;159(6):1027–1035.e3. doi: 10.1016/j.ajo.2015.02.008

Matrix metalloproteinase 9 promotes galectin-3 cleavage in vitro. (Upper panel) Quantification of MMP9 mRNA in conjunctival impression cytology samples from 10 normal subjects (10 eyes) and 15 dry eye patients (17 eyes) by qPCR. Data for individual samples are presented, with mean value indicated by horizontal bar for each group. rhMMP9 promoted galectin-3 cleavage in a concentration- and time-dependent manner (middle and lower panels, respectively), producing a 25-kDa cleavage product. Samples in the middle panel were incubated at 37 °C for 81 min. Samples in the lower panel were incubated at 37 °C with 1.0 ng/ μl rhMMP9. *P < .05.

Matrix metalloproteinase 9 promotes galectin-3 cleavage in vitro. (Upper panel) Quantification of MMP9 mRNA in conjunctival impression cytology samples from 10 normal subjects (10 eyes) and 15 dry eye patients (17 eyes) by qPCR. Data for individual samples are presented, with mean value indicated by horizontal bar for each group. rhMMP9 promoted galectin-3 cleavage in a concentration- and time-dependent manner (middle and lower panels, respectively), producing a 25-kDa cleavage product. Samples in the middle panel were incubated at 37 °C for 81 min. Samples in the lower panel were incubated at 37 °C with 1.0 ng/ μl rhMMP9. *P < .05.