Skip to main content
. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Am J Ophthalmol. 2015 Feb 19;159(6):1027–1035.e3. doi: 10.1016/j.ajo.2015.02.008

Figure 5.

Figure 5

Degradation of galectin-3 in human tears involves protease activity. Immunoblots demonstrating partial or complete degradation of endogenous galectin-3 in an in vitro assay using tears from three patients with dry eye. The broad-spectrum proteinase inhibitor cocktail complete™, but not the pan-specific MMP inhibitor GM6001, impaired cleavage of galectin-3 in samples from two patients. The presence of the latent (pro-MMP9) and active forms of MMP9 in these tear samples was confirmed by immunoblot. MMP9 blots were from the same tear samples used in the galectin-3 blots above.