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. Author manuscript; available in PMC: 2015 May 11.
Published in final edited form as: Curr Pharm Biotechnol. 2014;15(10):987–995. doi: 10.2174/1389201015666141031111916

Figure 4. Measurement of the apparent affinity of 2AR for serotonin using ion channel activity.

Figure 4

(A) Representative barium-sensitive traces of KIR2.3 currents obtained in oocytes expressing 2AR in response to increasing concentrations (0.1 nM to 1 mM) of serotonin (SER). (B) Dose-response curve showing Gq-mediated inhibition of KIR2.3 current elicited by serotonin in oocytes expressing 2AR. Response was normalized to the maximum value. (EC50 = 214 ± 0.01 nM).