FIG. 1.

Developing KB-R7785 chronic tympanic membrane (TM) perforations. Figure demonstrates the lack of progression, through the proliferation and migration of keratinocytes of the TM wound following perforation and application of KB-R7785 with staining with Hematoxylin and Eosin (a, c) and Cytokeratin (b, d). Time points are day 2 (a), showing the perforation edge (arrow) and surrounding exudate, day 7 (b), showing keratinization inhibited (arrow), and day 44 (c), showing a perforation (arrow) and no keratinization, day 44 (d) showing a lack of keratinocyte proliferation at the handle of malleus attachment to the TM (arrow). Scale bar=10 μm (magnification 10×). Color images available online at www.liebertpub.com/tea