FIG. 5.

The healed TM following HB-EGF treatment compared with healed controls. Figure shows Cytokeratin (brown) staining to identify the keratinocytes and keratin layers in the TM. The HB-EGF-treated chronic perforation (a) shows normal TM layers, including a thick keratin layer compared with the control TM (b). The HB-EGF-treated TM (a) has a normal thickness connective tissue layer compared with the lack of keratinization and thick disorganized connective tissue layer of the control TM (b). Scale bar=10 μm (magnification 40×). Color images available online at www.liebertpub.com/tea