Table 1.
Primers Used for Gene Amplification
Genes | Primers | Position in HXB2 | Primer sequence (5′-3′) | Direction |
---|---|---|---|---|
5′-half | U-LTR5-AE-new | 1–34 | 5′-GCGCCGAATTCTGGATGGGCTAATTTACTCCARGARAAGACAAGA-3′ | Forward (outer and inner) |
07Rev8-new | 5219–5185 | 5′-CCTARTGGGATRTGTACTTCTGARCTTACYTTTGG-3′ | Reverse (outer) | |
Rev11 | 5066–5041 | 5′-ATCATCACCTGCCATCTGTTTTCCAT-3′ | Reverse (inner) | |
3′-half | D-LTR3-AE | 9656–9691 | 5′-GGTCTGAGGGATCTCTAGTTACCAGAGTCC–3′ | Reverse (outer and inner) |
07For7 | 4875–4912 | 5′-CAAATTAYAAAAATTCAAAATTTTCGGGTTTATTACAG-3′ | Forward (inner) | |
VIF1 | 4900–4923 | 5′-GGGTTTATTACAGGGACAGCAGAG-3′ | Reverse (inner) |