FIG. 1.

Generation of Herc6 knockout mice. (A) Schematics of Herc6 knockout strategy. (B) Schematic diagram of Herc6 protein and the target deletion region is indicated with an arrow. (C) PCR genotyping of mouse tail DNA. (D) Southern blot analysis of mouse tail DNA. (E) Western blot analysis of protein ISGylation. Twenty-four hours after poly I:C injection, spleens were harvested. Herc6 protein was detected in the spleen extracts from WT mice, but not from Herc6 knockout mice by Western blotting using anti-mouse Herc6 polyclonal antibody. WT, wild type; He, heterozygous; KO, knockout.