FIG. 3.

Analysis of IFN and inflammatory response in Herc6 knockout cells. (A) BMDM from WT and Herc6 knockout mice were cultured in the presence of 100 U/mL of mIFN-β for the indicated time periods. Cells were harvested and subjected to Western blotting against pSTAT1, STAT1, and actin. The ratio of p-STAT1/total-STAT1 was also quantified as indicated. (B) Macrophages from WT and Herc6 knockout mice were treated with mock, SV at m.o.i. 10, or poly I:C 10 μg/mL. Twenty-four hours after treatment, cell culture media were harvested and subjected to ELISA for mIFN-β (upper) or mIL-6 (bottom). ELISA, enzyme-linked immunosorbent assay; m.o.i., multiplicity of infection; SV, Sendai virus.