Figure 3.

Mrg15 is required for Cap-H2 localization on polytene chromosomes. (A–C) Representative images of salivary gland polytene chromosomes from control (A), Cap-H2⁰⁰¹⁹ (B), and Mrg15 RNAi (C) larvae stained for Cap-H2 (red), Mrg15 (green), and DNA (blue). Scale bar, 10 μm. (D, E) Relative fluorescence intensity quantification showing significant decrease of Cap-H2 and Mrg15 in Cap-H2⁰⁰¹⁹ (D) and Mrg15 RNAi (E) polytene chromosomes. Mean fluorescence intensity was measured using four nuclei from each of three individual pairs of salivary glands and values were normalized to DAPI. *P < 0.05, **P < 0.01, two-tailed Student's t-test. Error bars, SEM.