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. 2015 Mar 9;5(5):803–817. doi: 10.1534/g3.115.016634

Figure 6.

Figure 6

Mrg-binding motif is required for Cap-H2-mediated unpairing of homologous chromosomes. Kc cells transiently expressing EGFP (A), Cap-H2-EGFP (B), Cap-H2-MBM-EGFP (C), Cap-H2-ΔC23-EGFP (D), or Cap-H2-ΔC23-MBM-EGFP (E) labeled with DNA FISH probes to the 2L (green) and X chromosomes (red). Scale bar, 2.5 µm. (F) Number of FISH spots per nucleus in Kc cells transfected as in (A–E). At least 145 cells were counted for each category shown. *P < 0.05, **P < 0.01, two-tailed Student's t-test. P values correspond to statistical significance relative to control, except where indicated by horizontal lines. Asterisks located above horizontal black line indicate significance between cells expressing Cap-H2-EGFP or Cap-H2-ΔC23-EGFP and the corresponding MBM mutant. Error bars, SEM; n.s. = nonsignificant.