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. 2015 Mar 9;5(5):803–817. doi: 10.1534/g3.115.016634

Figure 7.

Figure 7

Mrg-binding motif is required for Cap-H2-mediated axial compaction. (A) DNA-stained Kc cells displaying representative chromatin gumball phenotypes after overexpression of Cap-H2-ΔC23-EGFP. DNA-stained Kc cells transiently expressing EGFP (B), Cap-H2-EGFP (C), Cap-H2-MBM-EGFP (D), Cap-H2-ΔC23-EGFP (E), or Cap-H2-ΔC23-MBM-EGFP (F). (G) Percentage of Kc cells transfected as in (B–F) exhibiting the chromatin gumball phenotype. *P < 0.05, **P < 0.001, two-tailed Fisher’s exact test. Error bars represent 95% C.I.; a minimum of 500 cells were counted for each category. Kc cells transiently expressing EGFP (H), Cap-H2-EGFP (I), Cap-H2-MBM-EGFP (J), Cap-H2-ΔC23-EGFP (K), or Cap-H2-ΔC23-MBM-EGFP (L) labeled with FISH probes for two X chromosome loci, X chromosome probe 1 (X1, red) and X chromosome probe 2 (X2, green). Scale bar, 2.5 µm. (M) Pairwise distances were measured for X1–X2 FISH probes (n = 60). *P < 0.05, two-tailed Student's t-test. P values correspond to statistical significance relative to control, except where indicated by horizontal lines. Asterisks located above horizontal black line indicate significance between cells expressing Cap-H2-EGFP or Cap-H2-ΔC23-EGFP and the corresponding MBM mutant. Error bars, SEM.