Figure 3.

Conidial trehalose levels during germination on glucose and CA. The 5×10⁷ conidia from seven NPP deletion mutants and wild-type strain were germinated on minimal media plus glucose or CA, as a sole carbon source, for 150 min at 37°. The total protein lysates from conidia were used to measure the trehalose and were compared to standard curve. C = control (fresh conidia in water); Glu = glucose; CA = hydrolyzed casein. Presented is the average (± standard deviation) of three independent experiments. Significance = *P < 0.05, ** P < 0.01, and *** P < 0.001. The lines above the bars show the comparisons between the wild-type and the mutant controls or between the controls and Glu/CA treatments for each specific strain.