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ADP/ATP ratio. The seven NPP deletion mutants were grown on minimal media plus CA for 24 hr and transferred to minimal media plus glucose for another 24 hr. The total protein lysates (10 μg) from mycelia were used to quantify the ADP/ATP ratio using the ADP/ATP ratio assay kit (Sigma). Significance = *P < 0.05, **P < 0.01, and ***P < 0.001.
