Table 6. Summary of the phenotypic characterization of the A. nidulans phosphatase mutants.

	Fungal Strain
Trait	WT	ΔmsgA AN4544	ΔppsA AN0129	ΔpsrA AN10077	ΔptcD AN0914	ΔptcE AN5722	ΔptpB AN4896	ΔsdpA AN10138
Direct growth on glucose (Fig. 2)	+	−	−	−	−	−	−	−
After germination growth on glucose (Fig. 2)	+	+	+	+	+	+	+	+
Pre-CA and growth on glucose (Table 1)	0.056	0.033**	0.032**	0.033**	0.033**	0.033**	0.030***	0.035***
Isotropic growth on glucose (%) (Table 3)	202	75****	105**	26****	24****	0****	105****	46****
Cell cycle defect (Table 4)	N/A	G1-S	M	N/A	N/A	N/A	G1-S	N/A
Trehalose mobilization on Glucose (Fig. 3)	+	+	+	+	+	+	+	+
Trehalose mobilization on CA (Fig. 3)	+	+	−	−	−	−	−	−
Before germination 2-DG (mM) (Fig. S1)	0.08	0.1 (++)	0.1 (+)	0.08	0.1 (++)	0.1 (++)	0.05 (−)	0.08 (−)
After germination 2-DG (mM) (Fig. S2)	0.01	0.04 (++)	0.04 (++)	0.025 (+)	0.04 (++)	0.04 (++)	0.04 (++)	0.05 (+++)
Glucose consumption (% WT) (Fig. 4)	89.4	48.3**	103	63.3*	64.1*	72.6*	82.9	59.8*
Glucose respiration (% WT) (Fig. 4)	100	79.2***	42.4**	90.3	58.1**	49.8***	45.5*	89.1
Trehalose (μg trehalose/μg protein) (Fig. 6)	0.21	0.16**	0.22	0.06***	0.24	0.11***	0.12***	0.15**
Glycerol content (Fig. 6) (μmol/mg protein)	3.5	6.9***	3.3	4.1	8.3***	7.5***	2.0*	2.6
Pyruvate content (Fig. 6) (μmol/mg protein)	1.4	0.1***	1.3	4.3*	0.1*	0.3**	2.9***	3.1*
Glycolytic saccharide precursors (Fig. 2)	+	−	−	−	−	−	−	−
Nonsaccharide carbon sources (acetate, ethanol, glycerol) (Fig. 2)	+	−	−	−	−	−	−	−
Lipid/casein amino acids (Fig. 2)	+	+	+	+	+	+	+	+
Precursors for pyruvate (Fig. 7)	++	+	+	+	+	+	+	+
Precursors for acetyl-CoA or α-KGDH (Fig. 7)	+	−	−	−	−	−	−	−
Precursors pre-succinyl CoA (Fig. 7)	+	+	+	+	+	+	+	+
ADP/ATP ratio (Fig. 8)	0.6	3.4**	1.28	3.5*	2.8*	3.2**	3.4*	1.5*
Alpha-keto glutarate activity (Fig. 9) (nmol/mg.min)	5.9	0.1***	3.3*	1.8***	0.2***	3.0**	0.6***	0.7***

Level of statistical significance: *P < 0.05, **P < 0.01, and ***P < 0.001. Level and direction of alteration in growth: +/−.