Fig. 4.

Senju, a UDP-galactose transporter, is required for the synthesis of galactose-containing glycans. (A) Transportation of various nucleotide sugar substrates by Senju. *P < 0.05 (Student t test). (B) Lectin microarray analysis of lymph glands from dl/Dif mutants (J4, control) and senju¹ with a dl/Dif mutant background (J4, senju¹). The binding of senju¹ lysates to less and more reactive lectins is shown relative to that of control fly lysates. The specificities of the lectins are shown. The galactose moieties are highlighted in red. (C) Synthesis of N-linked glycans on Myc-tagged Spz and SPE in senju¹. Immunoblot analysis using an anti-Spz C106 antibody (Upper) or an anti-Myc antibody (Lower), respectively. The genotypes of larvae used in this study were srp-Gal4 > UAS-spz-myc and srp-Gal4 > UAS-spz-myc;senju¹, or srp-Gal4 > UAS-SPE-myc and srp-Gal4 > UAS-SPE-myc;senju¹. The SPE image was taken in the same blotted membrane under the same conditions, and the left half was flipped horizontally. (D) Drs expression in WT flies after the transfer of native or heat-denatured senju¹ or WT hemolymph [which was set as 1 (control)]. *P < 0.05 (Student t test).