Table 1.
Nuclear response to pulling in NIH 3T3 fibroblasts, SW13 adrenal carcinoma epithelial cells, and MEFs
| Just before detachment | At steady state after detachment | ||||
| Condition | n | Maximum nuclear deformation, % | Maximum nuclear translation, µm | Relaxation of nuclear deformation, % | Recovery of nuclear position, % |
| NIH 3T3 fibroblasts | |||||
| Control | 12 | 26 ± 3 | 1.32 ± 0.15 | 69 ± 4 | 121 ± 14 |
| CYTO-D | 10 | 28 ± 3 | 1.77 ± 0.19 | 65 ± 4 | 65 ± 4* |
| NOC | 10 | 26 ± 4 | 1.73 ± 0.31 | 60 ± 7 | 68 ± 2* |
| GFP-KASH4 | 10 | 26 ± 4 | 1.68 ± 0.29 | 56 ± 5 | 49 ± 6* |
| SCRAM | 13 | 21 ± 3 | 1.74 ± 0.35 | 67 ± 6 | 85 ± 6 |
| Vim siRNA | 25 | 38 ± 4† | 2.89 ± 0.57† | 76 ± 2 | 72 ± 5 |
| SS-GFP-KDEL | 12 | 22 ± 3 | 1.02 ± 0.24 | 61 ± 1 | 86 ± 11 |
| SS-HA-SUN1L-KDEL | 10 | 39 ± 6# | 1.75 ± 0.46 | 64 ± 3 | 66 ± 6 |
| SW13 adrenal carcinoma cells | |||||
| With vim | 11 | 20 ± 2 | 1.16 ± 0.27 | 59 ± 7 | 91 ± 22 |
| Without vim | 13 | 34 ± 4†† | 2.07 ± 0.33†† | 56 ± 3 | 62 ± 7†† |
| Without vim rescued | 8 | 20 ± 2 | 1.02 ± 0.17 | 50 ± 5 | 94 ± 5 |
| MEFs | |||||
| WT | 10 | 20 ± 2 | 0.97 ± 0.15 | 56 ± 6 | 102 ± 1 |
| Lmna−/− | 13 | 50 ± 5** | 2.37 ± 0.53** | 53 ± 3 | 49 ± 8** |
| GFP-KASH4 in WT | 9 | 15 ± 2 | 0.51 ± 0.04** | 57 ± 6 | 58 ± 7* |
Maximum nuclear deformation and the recovery of nuclear deformation was calculated with the approach in Fig. S10A. Maximum nuclear translation was calculated as the distance traveled by the back edge until nuclear detachment from the micropipette tip. Values are mean ± SEM. Force = 6 nN for NIH 3T3 fibroblasts and 4 nN for SW13 adrenal carcinoma cells and MEFs. Statistically significant differences between control and condition are indicated by symbols: *P < 0.05 relative to control; †P < 0.05 relative to SCRAM; **P < 0.05 relative to WT; #P < 0.05 relative to SS-GFP-KDEL; ††P < 0.05 relative to With vim. Control, NIH 3T3 cells; CYTO-D, NIH 3T3 cells treated with cytochalasin-D; GFP-KASH4, NIH 3T3 cells overexpressing GFP-KASH4; GFP-KASH4 in WT, wild-type MEFs overexpressing GFP-KASH4; Lmna−/−, MEFs lacking lamin A/C; NOC, NIH 3T3 cells treated with nocodazole; SCRAM, NIH 3T3 cells transfected with scrambled siRNA; SS-GFP-KDEL, NIH 3T3 cells inducibly expressing SS-GFP-KDEL; SS-HA-SUN1L-KDEL, NIH 3T3 cells inducibly expressing SS-HA-SUN1L-KDEL; Vim siRNA, NIH 3T3 cells transfected with vimentin siRNA; With vim, SW13 cells containing vimentin; Without vim, SW13 cells lacking vimentin; Without vim rescued, SW13 cells lacking vimentin on transfection with CFP-vimentin; WT, wild-type MEFs.