Fig. 2.
A charged head group and a polyunsaturated acyl tail are necessary. (A) Induced G(V) shifts for 70 μM of indicated substance. Means ± SEM n = 3–8. (B) pH dependence of the G(V) shift caused by the application of 70 µM DHA on Kv7.1. Means ± SEM. ΔVmax = −28.0 mV, c0.5 = 1.8 × 10−8 = pH 7.7. n = 3–5. (C) Concentration-response curves for DHA on Kv7.1 at different pHs. pH 9.0: ΔVmax = −27.7 mV, c0.5 = 10 μM; pH 7.4, see Fig. 1C. n = 3–8. (D) Schematic illustration of PUFA modulation. PUFAs incorporate in the lipid cleft close to the channel’s VSD and electrostatically affect the S4 movement. +++, S4 gating charges.