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. 2015 Apr 21;112(18):5714–5719. doi: 10.1073/pnas.1503488112

Fig. 3.

Fig. 3.

KCNE1 abolishes PUFA effect on Kv7.1/KCNE1 at physiological pH. (A–D) DHA (70 μM) applied on Kv7.1/KCNE1 at pH 7.4 (A and B) and pH 9 (C and D). DHA (●), control (○). Bold traces = 0 mV (A) or +10 mV (C). Dashed curve in D is control curve shifted −35 mV. (E) pH dependence for 70 µM DHA. Kv7.1/KCNE1: ΔVmax = −29.0 mV, c0.5 = 2.5 × 10−9 = pH 8.6. n = 3–6. For Kv7.1, see Fig. 2B. (F) Model: KCNE1 changes probability of protonation of negatively charged acidic (Upper) and positively charged amine (Lower) PUFA analogs, thereby changing PUFA-Kv7.1 voltage sensor (+++) interactions. (G) G(V) shift induced by 70 µM DHA or AA+. n = 3–8.