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. 2015 Apr 21;112(18):E2376–E2384. doi: 10.1073/pnas.1424241112

Fig. 5.

Fig. 5.

IFN-λ (PEG-IL-28A) suppresses B16F10 metastases in an NK cell-dependent fashion. (A and B) Groups of five WT mice in each panel were injected i.v. with B16F10 melanoma cells (2 × 105). Mice received PEG-IL-28A or mIFN-αβ (25 μg i.p.) daily (days 0–5). (C) Groups of 4–12 RAG2−/−γc−/− recipient mice were reconstituted for 5 d with 2 × 105 IL-28R−/− or WT NKs (sorted by TCRβneg, NKp46+, NK1.1+) and injected i.v. with B16F10 melanoma cells (2 × 105). Some mice received no NK cell transfer. Mice then received mock or PEG-IL-28A (25 μg i.p.) daily (days 0–5). In A, 14 d after tumor inoculation, the lungs of these mice were harvested and fixed, and the number of B16F10 colonies was counted under a dissection microscope. In B and C, survival of mice was plotted, and statistical analysis was performed by Mantel–Cox Log-rank test; *P < 0.05, **P < 0.01, ****P < 0.0001.