Fig. 3.

Symmetrical monobody block. Single Bpe channels were recorded under biionic conditions—500 mM NaF cis/NaCl trans at −25 mV holding voltage. (A) Representative traces from either side at indicated L3 concentrations. (B) Bimolecular kinetics of single-sided block. Effective rate constants 1/τ of binding or (Inset) dissociation are plotted against L3 concentration to show linear dependence of the on rate and constancy of the off rate. Filled and open circles indicate cis or trans monobody additions, respectively. Each point represents the mean and SEM of three to seven separate single-channel runs, except for points without error bars, which represent single runs.