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. 2015 Apr 20;112(18):5815–5820. doi: 10.1073/pnas.1504777112

Fig. 3.

Fig. 3.

Renal function and histology at age 40 wk in L/L Akita diabetic mice with Tgfb1 switched from low to high by podocyte-specific or proximal tubule-specific induction of Cre recombinase. (A) Urinary albumin excretion. (B) Urine volume. (C) Urine glucose excretion. (D) Plasma urea nitrogen concentration. (E) Plasma creatinine concentration. (F) Creatinine clearance. (G and H) Periodic acid-Schiff (PAS) staining with hematoxylin of the glomerulus. (Scale bar: 50 μm.) (G) L/L:A/+ mice with podocyte-specific switching (L/L:A/+ P). (H) L/L:A/+ mice with proximal tubule-specific switching (L/L:A/+ T). (I and J) Masson’s trichrome staining of renal cortex. (I) L/L:A/+ P. (J) L/L:A/+ T. (K) Percentage area of PAS-positive mesangial material per glomerular tuft. (L) Percentage of open capillary area per glomerular tuft. (M) Thickness of glomerular basement membrane (GBM). (N) Width of podocyte foot processes. (O) Thickness of tubular basement membrane (TBM) in proximal tubules. Bars are color coded to indicate switching: blue (L/L:A/+; no switching), pink (L/L:A/+ P, in podocytes), and turquoise (L/L:A/+ T, in tubules). *P < 0.05, **P < 0.01 vs. L/L:A/+ mice.