Fig. 4.

IVIG/F241A-activated T_reg cells efficiently suppress T cell-mediated autoimmunity in EAE mice. (A) C57BL/6 wild-type mice were immunized with MOG_35–55 peptide to induce EAE. Starting on day 5 post EAE induction, mice received i.v. injections of PBS, IVIG (1 g/kg), or NA-IVIG (1 g/kg) every 5 d. Clinical scores of EAE are shown. (B and C) Mice were euthanized and CD4⁺ effector T cells (B) and T_reg cells (C) from draining lymph nodes were analyzed by flow cytometry. (D) EAE was induced in C57BL/6 wild-type mice by immunization with MOG_35–55 peptide. Starting on day 5 and every 5 d thereafter, mice received nonsialylated F241A (0.033 g/kg) or PBS intravenously. For T_reg-cell depletion, mice were given the T_reg-cell depletion antibody PC61 (400 µg) 3 d before EAE induction as well as every fifth day postinduction. Clinical scores of disease are depicted. (E and F) Cells from draining lymph nodes of EAE mice were isolated and analyzed for the percentages of CD4⁺ effector T cells (E) and T_reg cells (F) by flow cytometry. Means ± SEM are plotted; *P < 0.05; **P < 0.01; ***P < 0.001 determined by Tukey’s post hoc test.