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. 2015 Apr 21;112(18):E2317–E2326. doi: 10.1073/pnas.1505569112

Fig. 3.

Fig. 3.

RAD23B recruitment follows OCT4/SOX2 binding. (A) Immunoblotting of D3 mESCs at different time points after lentiviral-mediated knockdown (KD) of OCT4. KD3 and KD4 constructs were chosen for further analyses. Uninfected cells (–) control for knockdown efficiency. (B) Oct4, Sox2, and Rad23b transcript levels in D3 mESCs at different time points after OCT4 KD compared with uninfected (–) cells. Values are average and SD of two independent experiments, normalized to Gapdh. (C) Binding of OCT4, SOX2, and RAD23B proteins at Oct4/Pou5f1, Nanog, and Klf4 enhancers in D3 mESCs at different time points after OCT4 KD, evaluated by ChIP-qPCR. Binding of Pol II and TBP on actin promoter (Actb) controls for cell viability and chromatin quality across time points. Plotted are average and SD from the two KD constructs in B.