Fig. 5.

The RNA-binding domains of hnRNP L are required for CSR. (A) CSR complementation assay performed by cotransfecting L11 cells with the siRNA-resistant WT hnRNP L construct (wt-L^R) and hnRNP L siRNA. The percentages of IgA switched cells are indicated in the FACS profiles. (B) CSR rescue efficiencies from three independent experiments. A representative Western blot analysis shows the degradation of endogenous but not exogenous hnRNP L (wt-L^R). (C) Representation of the various hnRNP mutants used in CSR complementation experiments. (The “Δ” with numbers indicates the specific RRM domain deleted.) (D) Summary of CSR rescue efficiencies of the hnRNP L mutants from three independent experiments. (E) Western blot analysis shows the protein expression associated with each of the L^R constructs.