Fig. 6.

RNA-dependent interactions of AID with hnRNP K and L. (A) Schematic view of the 3xFLAG-tagged WT AID and C- or N-terminal deletion mutants expressed in HEK293T cells and used in the PAR-CLIP assay. (B) Western blot analysis of the anti-Flag immunoprecipitations from 293T cells expressing WT or mutant FLAG-tagged AID and treated with 4-SU. The results show the association of the various forms of AID with hnRNP K and L and that the AID N terminus is required for their association. (C) RNase treatment of the cell lysates prevents AID association with hnRNPs. Neither hnRNP K nor hnRNP L coprecipitated with AID if the cell lysates were pretreated with RNase. (D) AID in association with an hnRNP (K or L) complex may edit specific RNAs and regulate DNA cleavage and recombination steps independently.