Fig. 6.

Disruption of antisense transcription and R-loop formation results in chromatin compaction and loss of NF-κB binding in the VIM gene promoter. (A) Fragments analyzed by qPCR in the VIM promoter. (B) Chromatin immunoprecipitation experiments with histone H3 antibody (H3) and control antibody (IgG) in SW480 cells. (C) Micrococcal nuclease accessibility assay on nuclei isolated from ASO-treated SW480 cells. (D) As in C, but comparing overexpression of RNASEH1 with empty vector. (E) Chromatin immunoprecipitation experiments with p65/RelA antibody or control (IgG) antibody in SW480 cells. (F) As in E, in ASO-treated cells. Levels were calculated relative to control samples. (G) As in F, but comparing overexpression of RNASEH1 with control-transfected cells. Throughout the figure, *P < 0.05; **P < 0.01; ***P < 0.005 from Student's t test.