Fig. 3.
Orai1 KO mice demonstrate normal mammary gland development and secretory activation but impaired milk let down. (A) Mammary whole mounts from lactating Orai1−/− mice versus control genotypes (days 1 and 4 of lactation) and cKO mice (Orai1fl/−;MMTV-Cre) versus control genotypes (day 4 lactation, n = 3). (Scale bar, 400 µm.) (B) H&E staining of mammary glands from Orai1−/− or control mice on gestation day 18 (i–iii), lactation day 1 (iv–vi), and lactation day 4 (vii–ix) (n = 2–3). [Scale bars, 300 µm (60 µm, higher magnification image).] Lu, alveolar lumen. Arrows, secretory epithelium; arrowheads, cytoplasmic lipid droplets. (C) Actin staining of myoepithelial structures with phalloidin and (D) Oxtr mRNA levels in lactating mammary glands (n = 3). Data represent mean ± SEM; P > 0.05, Student’s t test.