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. 2015 Mar 14;34(9):1214–1230. doi: 10.15252/embj.201489920

Figure 1. Che-1 inhibits mTOR activity.

Figure 1

  • A, B Western blot (WB) analysis of total cell extracts (TCEs) from HCT116 cells transiently transfected with siRNA GFP (siControl) or two different siRNA Che-1 (siChe-1-1 and siChe-1-2) and treated where indicated with the mTOR inhibitor CCI-779 (100 nM).
  • C Cell size analysis of HCT116 cells transiently transfected as in (A) and treated where indicated with CCI-779. Seventy-two hours after transfection, cell size was measured with a Coulter counter. The data represent the mean ± SD from three independent experiments performed in duplicate. *P < 0.03, **P < 0.001.
  • D WB analysis with the indicated Abs of TCEs from TSC2+/+ and TSC2−/− MEF cells transiently transfected with Myc-Che-1 or pCS2-MT control vector.
  • E WB with the indicated Abs of TCEs from ASM cells transiently transfected as in (D) and treated where indicated with 1 μM 5-Azacytidine (5-Aza).

Source data are available online for this figure.