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. 2015 Mar 12;34(9):1231–1243. doi: 10.15252/embj.201490578

Figure 3. FGFR1 and FGFR2 are expressed in adult cortical projection neurons.

Figure 3

  1. In situ hybridization of FGFR1 (top) and FGFR2 (bottom) mRNA in FGFR-competent mice. Scale bar equals 200 μm.
  2. In situ hybridization of FGFR1 (top) and FGFR2 (bottom) mRNA in forebrain FGFR1 (top)- and FGFR2 (bottom)-deficient mice. Scale bar equals 200 μm.
  3. Retrograde labeling of CST projection neurons with dextran conjugated with Texas Red® (green) shows that many of these neurons express FGFR1 (red, top) and FGFR2 mRNA (red, bottom; insets in top and bottom panels are twofold magnification of boxed areas). Scale bar equals 100 μm (20 μm in insets).
  4. Quantification of the percentage of CST projection neurons in layer V of the cortex expressing FGFR1 (white bar) and FGFR2 mRNA (gray bar; n = 5 animals per group). Mean ± SEM.
  5. Quantification of the intensity of the in situ signal for FGFR1 mRNA in FGFR2-deficient mice (green bar) and FGFR2 mRNA in FGFR1-deficient mice (blue bar) normalized to the signal intensity measured in the respective FGFR-competent control group (white bars; n = 3 animals per group). Mean ± SEM. No significant differences between the groups were detected (unpaired two-tailed t-tests).