Table 1.
Technical validation of the EL-NE assay
| Technical validation step | EL-NE |
|---|---|
| Detection range | 0.90 nM – 119 nM |
| Lower limit of quantification | 1.9 nM |
| Intra-assay variation | 7% |
| Inter-assay variation | 11% |
| Dilution range of serum samples | 1:2 (recommended) |
| Dilution range of plasma samples | 1:2 (recommended) |
| Dilution recovery in serum | 104% |
| Dilution recovery in plasma (Heparin) | 86% |
| Dilution recovery in plasma (Citrate) | 108% |
| Freeze-thaw recovery in serum | 96% |
| Freeze-thaw recovery in plasma (Heparin) | 87% |
| Freeze-thaw recovery in plasma (Citrate) | 104% |
| Analyte stability serum (24 h, 4°C/20°C) | 88%/77% |
| Analyte stability plasma (Heparin) (24 h, 4°C/20°C) | 95%/111% |
| Analyte stability plasma (Citrate) (24 h, 4°C/20°C) | 85%/77% |