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. 2015 May 11;5:10097. doi: 10.1038/srep10097

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In vitro SUMOylation of TbCentrin3-HA and TbCentrin3 K0-HA in the presence or absence of ATP TbCentrin3 K0-HA was the mutant in which all the lysine residues were mutated to arginine except the deleted C-terminal lysine K164 and K165. The samples were fractionated by SDS-PAGE (12%) and analyzed by immune blotting using anti-HA antibody. SUMOylated Tbcentrin3-HA is indicated by asterisk (*).