Figure 1. TRIM23 is expressed during adipocyte differentiation.
(A) Real-time PCR analysis of mRNA expression of Trim23 during 3T3-L1 differentiation. Total RNA was isolated from 3T3-L1 cells on the indicated days. Trim23 mRNA was normalized to that of Gtf2b. (B) Immunoblot analysis of TRIM23 and PPARγ protein during 3T3-L1 cell differentiation. (C) Real-time PCR analysis of mRNA expression of Trim23 in mouse adipose tissue. Trim23 mRNA was normalized to that of Gtf2b. (D) Subcellular localization of TRIM23. Nuclear extracts and cytoplasmic S100 fraction were prepared from 3T3-L1 cells, and immunoblot analysis of TRIM23, HDAC1, and GAPDH was performed.