Figure 4. TRIM23 knockdown does not affect the induction and occupancy of C/EBPβ and C/EBPδ at the Pparg promoter during adipogenesis.
(A) RNA levels of Trim23, Cebpb and Cebpd were determined by real-time PCR at 0, 6, 12, 24 and 48 hr. Expression level of each gene was normalized to that of Gtf2b. (B) Schematic representation of the Pparg promoters. The locations of the sequences amplified in the ChIP are shown at the bottom in base pairs relative to the Pparg1 and Pparg2 transcriptional start sites. (C) Occupancy of C/EBPβ and C/EBPδ on a subset of target genes at 4 hr. Ct values of each ChIP were normalized to that of input. (D) Cell proliferation analysis of 3T3-L1 cells during adipocyte differentiation. 3T3-L1 cells were counted at each time point. The data represent means ± s.d. from three independent experiments. The p values for the indicated comparisons were determined by Student's t test (*, p < 0.05; **, p < 0.01).