Figure 4. ISRIB enhances the thermo-stability of the regulatory subunit of eIF2B.
Clarified HEK293 cell lysates were treated with DMSO (-ISRIB) or with 200 nM ISRIB (+ISRIB) for 20 min. Treated and untreated lysates were partitioned into smaller aliquots and heated to different temperatures for 3 min and then centrifuged to remove precipitated proteins. The supernatant fraction was loaded onto a SDS-PAGE gel and immunoblotted with the indicated antibodies. The red asterisk indicates a background band that cross-reacts with the eIF2B4 antibody. Shown is a representative blot (N = 3).