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. 2015 May 11;5:10199. doi: 10.1038/srep10199

Figure 3. Both VosA and VelB bind to the fksA promoter in conidia.

Figure 3

(A) Schematic presentation of the promoter region of fksA. The black box (the −1,296 ~ −1,604 region of the fksA promoter) and the gray box (a region of the fksA ORF) represents the regions subject to PCR (shown in B). The 11-nucleotide sequence predicted to be recognized by VosA is shown. (B) Results of VosA-ChIP-PCR: the PCR amplicons separated on a 2% agarose gel are shown in the bottom panel. The input DNA before immuno-precipitation (IP) was used as a positive control (input). The chromatin extract being incubated with bead only (without anti-FLAG antibody) was used as a negative control (NC). The samples of THS30 lacking FLAG-tagged VosA or VelB were used as negative controls, too. Representative results are shown (measured in duplicates). All gel images were cropped (indicated by the red lines) to show the relevant data only. The ChIP-PCR amplified samples were separated in the same agarose gel. The full-length gels are presented in Supplementary Fig. S1A online.