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. 2015 May 7;2(4):e107. doi: 10.1212/NXI.0000000000000107

Figure 2. Brain-infiltrating immune cells mainly consist of CD8+ memory T cells.

Figure 2

Immunofluorescence staining of brain-infiltrating immune cells. All nuclei are stained with 4′,6-diamidino-2-phenylindole (white). Green and red dyes were used. Double-positive cells are therefore shown in yellow. Scale bars 50 µm. (A) Double staining for CD3 (green) and CD8α (red). Most CD3+ T cells coexpress CD8α. (B) CD8+ (red) T cells outnumber CD4+ (green) T cells. (C) Low numbers of CD45RA+ (green) CD8+ (red) double-positive T cells in multiple sclerosis (MS) brain tissue. (D) Many CD8+ T cells (red) coexpress CD45RO (green). (E) Naive T cells double-positive for CCR7 (red) and CD45RA (green) are mostly found within blood vessels and are barely detectable in the parenchyma of MS CNS. (F) Effector memory (CD45RO+CCR7−) (green arrow) and central memory (CD45RO+CCR+) (yellow arrow) T cells in MS lesions.