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. 2015 May 11;5:10159. doi: 10.1038/srep10159

Figure 7. LncRNA GAS5 mutant fails to arrest cell cycle at the G1 phase.

Figure 7

(a) Western blot detected YBX1 from GAS5-DEL and GAS5-FL RNA pull-down complexes. GAS5-DEL was biotin labeled lncRNA GAS5 mutant with its exon 12 deletion. GAS5-FL was biotin labeled full length lncRNA GAS5. NS was the biotin labeled non-sense RNA with similar length to GAS5, NC was the lncRNA GAS5 without biotin label. (b) The protein level of p21 and YBX1 after transfection with GAS5-E, GAS5-D or GAS5-B plasmids. (c) and (d) The cell cycle alteration after transfecting GAS5-E, GAS5-D or GAS5-B plasmids in HGC-27 (c) and in SGC-7901 (d). GAS5-E was the lncRNA GAS5 expression plasmid, GAS5-D was the lncRNA GAS5 mutant with its exon 12 deleted plasmid, and GAS5-B was the lncRNA GAS5 blank empty plasmid. *, p < 0.05.