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. 2015 May 11;5:9960. doi: 10.1038/srep09960

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Copyright © 2015, Macmillan Publishers Limited

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DBM and day 10 EBM structure and composition were examined macroscopically (left panel, scale bar = 1 mm) and microscopically (center panel: H&E and right panel: modified MMAB, scale bar = 100 μm) to reveal their material architectures and collagen content (a). EBM derived from EBs without or with βGP-treatment started on day 5 of EB formation was harvested at D5, D10, and D14. Growth factors extracted from both EBM and DBM were quantified by ELISA (b). The error bars represent standard error of the mean. n = 3 samples, Box-Cox transformation, ANOVA, Tukey’s post-hoc test, ***p < 0.005 compared to DBM, #p < 0.05 for marked comparison, %p < 0.05, %%p <0.01 compared to Day 14 +βGP EBM. ND: not detectable.