Table 2. Survival (%) of the wild-type host strain E.
| Strain |
Survival of cells in infected culture (% of survivors)
|
|||||
|---|---|---|---|---|---|---|
|
Phage Φ24
B
|
Phage Φ24
B
∆24
B
_1
|
|||||
| m.o.i.=1 | m.o.i.=5 | m.o.i.=10 | m.o.i.=1 | m.o.i.=5 | m.o.i.=10 | |
| MG1655 | 71 ± 9 | 49 ± 9 | 65 ± 8 | 61 ± 12 | 22 ± 15 | 31 ± 0 |
| MG1655 [pUC18] | 85 ± 6.3 | 52 ± 6.3 | 75 ± 8.3 | 77 ± 2.1 | 21 ± 4.0 | 17 ± 3.8 |
| MG1655 [pUC18_24B1] | nt | nt | nt | 81 ± 2.1 | 50 ± 8.3 | 60 ± 2.1 |
coli MG1655, MG1655 [pUC18] and MG1655 [pUC18_24B1] after infection with Φ24B and Φ24B∆24B_1 bacteriophages. Mean values from three independent experiments ± SD are shown. Statistical analysis (t test) was performed for results from each m.o.i. and indicated significant differences (P < 0.05) between fractions of bacterial cells surviving the infection with Φ24B and Φ24B∆24B_1 at m.o.i = 5 and 10 in most cases except the variants of complementation (MG1655 or MG1655 [pUC18] hosts infected with Φ24B versus MG1655 [pUC18_24B1] infected with Φ24B∆24B_1) where no significant differences (P > 0.05) were found. Differences in the mean fold change values for m.o.i = 1 between tested bacteriopohages did not reach statistical significance in the t test with P > 0.05. nt – not tested.