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. 2014 Aug 19;23(1):79–88. doi: 10.1038/mt.2014.126

Figure 1.

Figure 1

Expression profiles of miR-122 and miR-151. (a) Endogenous miR-122 levels in primary human hepatocytes (HH), HCC and non-HCC cell lines. (b) Endogenous miR-151 levels. (c) Schematic illustrations of BV vectors, the expressed miR-151 sponge and target miRNA. (d) miR-122 levels after BV transduction. (e) miR-151 levels after BV transduction. The miR-122/miR-151 levels were determined by TaqMan qRT-PCR using U6 small nuclear RNA as an internal control. For endogenous expression, the data were normalized to those in HH. For miRNA levels after BV transduction, the data were normalized to those of mock-transduced cells. All data represent the means ± SD of three independent culture experiments. CMV, CMV promoter; W, WPRE (woodchuck hepatitis virus post-transcriptional regulatory element) sequence; pA, polyadenylation signal; d2EGFP, destabilized green fluorescent protein.