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. 2014 Sep 16;23(1):171–178. doi: 10.1038/mt.2014.156

Figure 3.

Figure 3

EphA2-ENG T cells redirect bystander T cells to tumor cells. (a) A549 cells were cocultured with or without NT T cells and media of NT T cells or EphA2-ENG T cells. After 24 hours, IFN-γ production was determined by enzyme-linked immunosorbent assay (mean ± SD; n = 4). (b) Scheme of coculture transwell assays. (c) 1 × 106 NT T cells and 2.5 × 105 U373 cells were plated in the bottom well, and EphA2-ENG T cells in the insert well. The number of plated EphA2-ENG T cells ranged from 103 to 106. CD19-ENG T cells in the insert well and bottom wells without NT T cells served as controls. After 48 hours, live U373 cells were visualized by crystal violet staining. The experiment was performed three times and results of one representative experiment are shown.