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CART-33 copies persistent in the peripheral blood and the bone marrow as assessed by quantitative polymerase chain reaction (Q-PCR). Quantitative real-time PCR was performed on genomic DNA harvested from the patient's PBMNCs and bone marrow collected before and at the indicated serial time points after the CART-33 cell infusion. Primers specific for the transgene were used. CART, chimeric antigen receptor-modified T cells; PBMNC, peripheral blood mononuclear cell.
